Several equipments are available for students and researchers of the Cell and Molecular Biology Graduate Program at UFPR and Carlos Chagas Institute to carry out their research projects.

Center for Advanced Fluorescence Technologies

Equipments:

• In vivo imaging system for capturing internal images of live animals (fluorescence, luminescence and X-rays)
• Inverted fluorescence microscope (Zeiss) for the study of living cells
  
• Histological slides scanner (Zeiss) for capturing images of light and dark fields, phase contrast, DIC and fluorescence
• Multifotonic intravital confocal microscope (Nikon) and inverted confocal microscope (Nikon) for long-term study of live cells
• Super Resolution Fluorescence Microscope (Nikon)
• Confocal Microscope (Bio-Rad, Bioradiance 2100)
• Fluorescence microscope (Olympus, BX51)
• Flow cytometer (FACSCalibur and FACSMelody)

E-mail: ctaf.ufpr@gmail.com
Phone: +55 (41) 3361-1584
Location: Sector of Biological Sciences, UFPR
Opening: 8-12 h and 13-17h

Electron Microscopy Center

Equipments:

• Transmission Electron Microscope (JEOL JEM 1200EX-II)
• Scanning Electron Microscope (JEOL JSM 6360-LV)
• Scanning Electron Microscope (TESCAN VEGA3 LMU)
• Scanning Electron Microscope (FEI Quanta 450 FEG)
• Confocal Raman Microscope (Witec alpha 300R)
• Bench Scanning Electron Microscope (FEI Phenom)

Phone: +55 (41) 3361-3493
Location: Polytechnic Center, UFPR
Opening: 8h30min – 19h30min

Laboratories of the Graduate Program

Equipments:

• Ultramicrotome and knifemaker – for preparation of biological samples for transmission electron microscopy
• Cryostat and microtome – for preparation of biological samples for histology
• Class II laminar flow hoods, CO2 incubators, microbiological incubators, inverted microscopes, centrifuges and ultracentrifuge – for cell culture
• Thermocycler, master mix equipment, horizontal and vertical gel systems – for basic studies on RNA and proteins
• Refrigerated centrifuges and microplate readers (absorbance, fluorescence and luminescence) – for biochemical and quantification analysis

Location: Cell Biology Department, UFPR

Plataforms of Carlos Chagas Institute, Fiocruz – PR

Plataforms:

• Flow cytometry – FACSCanto II and a FACArea II
• Microscopy – confocal fluorescence microscope (Leica SP5) and a transmission electron microscope (JEOL) and a scanning electron microscope (JEOL)
• Mass spectroscopy – nanoLC chromatographs and mass spectrometer (Orbitrap)
• Protein engineering platform – ÄKTA (UPC and Pure), spectrophotometer (circular dichroism and luminescence), semi-automated protein crystallization system and stereoscopic microscope for analysis of crystals
• DNA analysis – SOLiD4, Ion PGM and Ion Proton equipment.

Access to these plataforms requires the registration of the research project and previous contact with the chair of each platform. Schedule obeys the same rules established for FIOCRUZ researchers.

Phone: +55 (41) 3316-3230
Location: Carlos Chagas Institute, Fiocruz – PR